Scott W N, Reich I M, Brown J A, Yang C P
J Membr Biol. 1978;40 Spec No:213-20. doi: 10.1007/BF02026007.
Using double-labeled isotope techniques, it can be shown that aldosterone induces the synthesis of several proteins in the mitochondria-rich (MR) cells of the toad's urinary bladder. Induced proteins have been identified both in the plasma membrane (mol wt = 170,000, 85,000 and 12,000) and the cytosol (mol wt = 36,000, 12,000 and 6,000) fractions of these mucosal cells. We have also shown that aldosterone (Aldo) induces the synthesis of a class of RNA having the properties of messenger RNA (mRNA). mRNA isolated from Aldo-treated mucosal cells was used as template in a cell-free protein-synthesis system prepared from rabbit reticulocytes. Preparations charged with mRNA from Aldo-treated cells synthesized two proteins that were not labeled when mRNA from control tissues was used as template. The electrophoretic mobility of one of these proteins was similar to an Aldo-induced membrane protein (mol wt = 70,000) found in the intact tissue.
运用双标记同位素技术能够表明，醛固酮可诱导蟾蜍膀胱富含线粒体（MR）的细胞中多种蛋白质的合成。在这些黏膜细胞的质膜（分子量分别为170,000、85,000和12,000）和胞质溶胶（分子量分别为36,000、12,000和6,000）组分中均已鉴定出诱导蛋白。我们还表明，醛固酮（Aldo）可诱导一类具有信使核糖核酸（mRNA）特性的核糖核酸的合成。从经醛固酮处理的黏膜细胞中分离出的mRNA，在由兔网织红细胞制备的无细胞蛋白质合成系统中用作模板。用经醛固酮处理的细胞的mRNA进行制备时，合成了两种蛋白质，而以对照组织的mRNA作为模板时这两种蛋白质未被标记。其中一种蛋白质的电泳迁移率与在完整组织中发现的一种醛固酮诱导的膜蛋白（分子量 = 70,000）相似。
