Grubhoffer L, Tichá M, Kocourek J
Biochem J. 1981 Jun 1;195(3):623-6. doi: 10.1042/bj1950623.
The lectin of the seeds of hairy vetch (Vicia villosa Roth), which selectively binds murine cytotoxic T-lymphocytes, was purified by simple affinity-chromatographic procedures on two different N-acetyl-alpha-D-galactosaminyl-carriers. The lectin thus obtained is homogeneous on polyacrylamide-gel electrophoresis both in acid and alkaline media and has a mol. wt. of approx. 120000. The lectin molecule appears to comprise four subunits of equal electrophoretic mobility, contains 4.3% of covalently bound neutral sugar and 0.72 Mn and 0.94 Zn atoms respectively. The anti-(blood-group A1) specific erythroagglutinating activity of the lectin can be detected at a limit concentration of 15 microgram/ml and is inhibitable most effectively by N-acetyl-D-galactosamine.
毛苕子（Vicia villosa Roth）种子中的凝集素能选择性结合小鼠细胞毒性T淋巴细胞，通过在两种不同的N-乙酰-α-D-半乳糖胺载体上进行简单的亲和层析程序将其纯化。如此获得的凝集素在酸性和碱性介质的聚丙烯酰胺凝胶电泳中均呈均一状态，分子量约为120000。凝集素分子似乎由四个具有相同电泳迁移率的亚基组成，含有4.3%的共价结合中性糖，分别含有0.72个锰原子和0.94个锌原子。该凝集素的抗（血型A1）特异性红细胞凝集活性在浓度低至15微克/毫升时即可检测到，并且最有效地被N-乙酰-D-半乳糖胺抑制。
