Agureev A P, Altukhov N D, Mokhova E N, Savel'ev I A
Biokhimiia. 1981 Nov;46(11):1945-56.
The rates of external and internal pathways of NADH oxidation in mitochondria of liver and skeletal muscle and skeletal muscle homogenate were studied at decreased pH and increased concentrations of Pi and CaCl2. The results obtained are indicative of an existence of an external pathway of NADH oxidation, which is insensitive to antimycin A and rotenone in skeletal muscle mitochondria. In the mitochondria of liver and skeletal muscle NADPH does not replace NADH in the external pathway; the NADH oxidation is sensitive to mersalyl and KCN. The decrease of pH of the incubation medium from 7.5 to 6.5 or the increase in Pi concentration up to 8-10 mM activates the external pathway and inhibits the internal pathway in liver mitochondria; the same effect is observed after addition of 10 microM CaCl2, pH 7.2. The external pathway of NADH oxidation in liver mitochondria is sensitive to the inhibitors of phospholipase A2 (nupercaine, EGTA) and the inhibitor of lipid peroxidation (ionole).
在pH降低以及磷酸（Pi）和氯化钙（CaCl2）浓度升高的情况下，对肝脏、骨骼肌线粒体以及骨骼肌匀浆中NADH氧化的外部和内部途径的速率进行了研究。所得结果表明存在NADH氧化的外部途径，该途径对骨骼肌线粒体中的抗霉素A和鱼藤酮不敏感。在肝脏和骨骼肌的线粒体中，NADPH不能在外部途径中替代NADH；NADH氧化对汞撒利和氰化钾敏感。将孵育介质的pH从7.5降至6.5，或将Pi浓度提高至8 - 10 mM，可激活肝脏线粒体中的外部途径并抑制内部途径；在添加10 microM CaCl2（pH 7.2）后也观察到相同的效果。肝脏线粒体中NADH氧化的外部途径对磷脂酶A2抑制剂（纽白卡因、乙二醇双乙醚二胺四乙酸）和脂质过氧化抑制剂（异壬醇）敏感。
