[Anticoagulating activity and lytic action of the heparin-antithrombin III and antithrombin III-heparin-thrombin complexes on non-stabilized fibrin].

The heparin-antithrombin III and antithrombin III-heparin-thrombin complexes were prepared by rapid mixing of the components at different weight ratios (1:1, 1:3, 1:4, 1:6, 1:7, 3:1 - heparin/antithrombin III), pH 7.0-7.2. The reaction mixture was incubated for 15 min at 37 degrees. The complexes were precipitated at pH 4.5-4.8. All the heparin-antithrombin III complexes possess high anticoagulating and lytic activities. The antithrombin III-heparin-thrombin complex prepared at the weight ratio of 1:1:6 has a high lytic activity. The antithrombin III-heparin-thrombin complex prepared at the weight ratio of 1:1:1 reveals a high anticoagulating activity and a strong lytic action with respect to the non-stabilized fibrin.