Asaka M, Nagase K, Miyazaki T, Shiraishi T, Alpert E
Clin Chim Acta. 1981 Dec 24;117(3):289-96. doi: 10.1016/0009-8981(81)90116-9.
A radioimmunoassay was developed for the direct quantification of aldolase A in human serum. The method is a double antibody radioimmunoassay using radioiodinated aldolase A4 homopolymer as ligand, chicken antibodies to aldolase A, and rabbit antibodies to chicken IgG. The lowest measurable amount by this method was 2 ng (0.01 U). The radioimmunoassay was shown to be specific for the aldolase A subunit, with no cross-reactivity with human aldolase B subunits or homopolymeric human aldolase C (C4). The immunoreactive aldolase A in the sera of 41 normal healthy subjects ranged from 130 to 210 ng/ml (0.81-1.31 U/1), with a mean of 171 /+- 39 ng/ml.
已开发出一种用于直接定量人血清中醛缩酶A的放射免疫测定法。该方法是一种双抗体放射免疫测定法，使用放射性碘化醛缩酶A4同聚物作为配体、针对醛缩酶A的鸡抗体以及针对鸡IgG的兔抗体。此方法可测量的最低量为2 ng（0.01 U）。该放射免疫测定法对醛缩酶A亚基具有特异性，与人醛缩酶B亚基或人醛缩酶C同聚物（C4）无交叉反应。41名正常健康受试者血清中的免疫反应性醛缩酶A范围为130至210 ng/ml（0.81 - 1.31 U/1），平均值为171±39 ng/ml。
