Segerson E C, Getz W R, Johnson B H
J Anim Sci. 1981 Nov;53(5):1360-7. doi: 10.2527/jas1981.5351360x.
A study was conducted with 24 crossbred boars (77.5 +/- 2.8 days of age) to determine the effects of low Se status on various spermatozoal characteristics and on Se concentration in semen, serum and primary and accessory reproductive tissues. All boars were fed a low Se diet (cornstarch and Torula yeast) ad libitum. Twelve boars were injected every 14 +/- 1 days with sodium selenite (.33 mg Se/kg body weight) and 12 served as saline-treated controls (low Se status). At 210 +/- 5 days of age, six boars in each group were slaughtered, and serum and various tissues were collected and assayed for Se. Treated boars had higher concentrations of Se in the serum (P less than .001), kidney (P less than .001), liver (P less than .001), heart (P less than .001), skeletal muscle (P less than .01), testis (P less than .01), epididymis (P less than .05), seminal vesicle (P less than .01), bulbourethral gland (P less than .001) and prostate (P less than .001) tissues. Starting at 230 +/- 4 days of age, semen samples were collected from the remaining boars at 4- to 6-day intervals until a total of four ejaculates had been obtained from all but two boars. There were no significant treatment differences in semen volume, percentage normal spermatozoa, percentage viability or spermatozoa concentration/milliliter; however, for combined semen Se data, treated boars had more Se than control boars in the whole semen (.165 vs .07 ppm, respectively), spermatozoa (.418 vs .199 micrograms/10(9) spermatozoa, respectively) and seminal plasma (.03 vs .007 ppm, respectively). The boars were castrated around 250 days of age, and no differences in testis length, diameter, weight and spermatozoal concentration were found between groups. Additionally, there were no apparent differences in daily gain, daily feed consumed and the feed to gain ratio between control and treated boars. Although concentrations of Se in serum, semen and reproductive tissues were much lower in control boars than in treated boars, no apparent impairment of sperm morphology or viability resulted from low Se status.
以24头杂交公猪（77.5±2.8日龄）进行了一项研究，以确定低硒状态对各种精子特征以及精液、血清和生殖主、副组织中硒浓度的影响。所有公猪自由采食低硒日粮（玉米淀粉和产朊假丝酵母）。12头公猪每隔14±1天注射亚硒酸钠（0.33毫克硒/千克体重），12头作为生理盐水处理的对照（低硒状态）。在210±5日龄时，每组屠宰6头公猪，采集血清和各种组织并测定硒含量。处理过的公猪血清（P＜0.001）、肾脏（P＜0.001）、肝脏（P＜0.001）、心脏（P＜0.001）、骨骼肌（P＜0.01）、睾丸（P＜0.01）、附睾（P＜0.05）、精囊（P＜0.01）、尿道球腺（P＜0.001）和前列腺（P＜0.001）组织中的硒浓度较高。从230±4日龄开始，每隔4至6天从其余公猪采集精液样本，直到除两头公猪外的所有公猪共获得四份射精样本。在精液体积、正常精子百分比、活力百分比或每毫升精子浓度方面，处理组之间没有显著差异；然而，对于合并的精液硒数据，处理过的公猪在全精液（分别为0.165和0.07 ppm）、精子（分别为0.
