Segerson E C, Johnson B H
J Anim Sci. 1980 Aug;51(2):395-9. doi: 10.2527/jas1980.512395x.
Selenium, (Se) content of selected tissues and semen and various spermatozoa parameters were evaluated in yearling Angus bulls given supplemental Se. Twenty-four bulls were allotted to two treatment groups: control (n-12) and Se (n-12). Se bulls were given an initial 10ml IM injection containing 50 mg of Se, then injections of 30 mg Se at 21-day intervals for 150 days. Pooled Se concentration (ppm) in serum collected at 21-day intervals averaged .01 and .08 (P < .001) for control and Se bulls, respectively. After 5 months of supplementation, eight bulls in each group were electroejaculated, and semen was processed and frozen for subsequent examination. Bulls were slaughtered for collection of testes, epididymides, seminal vesicles, kidney and liver tissues. Se (ppm) in kidney, liver, seminal vesicle, testis, caput, corpus and cauda epididymis of control and Se-treated bulls was, respectively: .84 and 1.27 (P < .005); .1 and .37 (P < .001); .1 and .21 (P < .001); .35 and .42 (P < .005); .39 and .44; .31 and .34, and .71 and .78. Se (ppm) in extended whole semen, the supernatant fraction and sperm pellet fraction from control and Se-treated bulls was, respectively: .07 and .16 (P < .004); .04 and .13 (P < .001), and .03 and .03. Percentage viability of thawed spermatozoa for control and Se-treated bulls was 26.5 and 23.1, respectively. No differences were observed between control and Se-treated bulls with respect to sperm concentration in the testis and caput, corpus and cauda epididymis. Se treatment of yearling Angus bulls increased the Se concentration of various tissues but had no apparent influence on sperm cell viability, number or Se concentration.
对补充了硒的一岁安格斯公牛的选定组织、精液中的硒（Se）含量以及各种精子参数进行了评估。将24头公牛分配到两个处理组：对照组（n = 12）和硒处理组（n = 12）。硒处理组的公牛最初肌肉注射10毫升含50毫克硒的溶液，然后每隔21天注射30毫克硒，持续150天。每隔21天采集的血清中合并硒浓度（ppm），对照组和硒处理组分别平均为0.01和0.08（P < 0.001）。补充5个月后，每组8头公牛进行电刺激采精，精液经过处理后冷冻以备后续检查。公牛被屠宰以采集睾丸、附睾、精囊、肾脏和肝脏组织。对照组和硒处理组公牛的肾脏、肝脏、精囊、睾丸、附睾头、附睾体和附睾尾中的硒（ppm）分别为：0.84和1.27（P < 0.005）；0.1和0.37（P < 0.001）；0.1和0.21（P < 0.001）；0.35和0.42（P < 0.005）；0.39和0.44；0.31和0.34，以及0.71和0.78。对照组和硒处理组公牛的稀释后全精液、上清液部分和精子沉淀部分中的硒（ppm）分别为：0.07和0.16（P < 0.004）；0.04和0.13（P < 0.001），以及0.03和0.03。解冻后精子的活力百分比，对照组和硒处理组分别为26.5和23.1。在睾丸以及附睾头、附睾体和附睾尾中的精子浓度方面，对照组和硒处理组之间未观察到差异。对一岁安格斯公牛进行硒处理可提高各种组织中的硒浓度，但对精子活力、数量或硒浓度没有明显影响。
