Alonso-Fernández J R, Bóveda M D, Parrado C, Peña J, Fraga J M
J Chromatogr. 1981 Nov 6;217:357-66. doi: 10.1016/s0021-9673(00)88090-5.
A procedure for the analysis of sugars of clinical interest in samples of urine impregnated on Whatman 3MM paper is described. The sugars are eluted from the sample and spotted directly on to the application zone of concentration-zone silica gel plates. followed by continuous development. The optimal composition of the eluent and developing solvent and the optimal development distance were established, together with the development time and its influence of the RF values. The locating reaction is based on the reduction of vanadium (V) to vanadium (IV) in acidic medium.
本文描述了一种用于分析浸渍在沃特曼3MM滤纸上的尿液样本中具有临床意义的糖类的方法。糖类从样本中洗脱后，直接点样到浓缩区硅胶板的加样区，随后进行连续展开。确定了洗脱剂和展开溶剂的最佳组成、最佳展开距离、展开时间及其对比移值的影响。定位反应基于在酸性介质中将钒（V）还原为钒（IV）。
