Nunez E A, Gershon M D, Silverman A J
J Histochem Cytochem. 1981 Nov;29(11):1336-46. doi: 10.1177/29.11.7320495.
Serotonergic mechanisms were examined in the bat pituitary. A seasonal fluctuation in the concentration of serotonin in the pituitary was found; the concentration rose just prior to hibernation, fell during hibernation, and transiently rose at the time of arousal from hibernation. This pattern is consistent with serotonin being stored within a cell that becomes inactive while bats hibernate. The uptake of 3H-serotonin was examined utilizing electron microscopic radioautography and the simultaneous light microscopic radioautographic localization of 3H-serotonin and the immunocytochemical localization of adenohypophyseal peptides. Uptake of 3H-serotonin was found to be a saturable process, inhibited by fluoxetine, but neither mimicked nor inhibited by norepinephrine. Cells responsible for uptake of 3H-serotonin contained luteinizing hormone immunoreactivity and were identified as gonadotrophs. The seasonal fluctuation and the presence of a specific serotonin uptake mechanism suggests that serotonin plays a role in the function of the adenohypophysis.
对蝙蝠垂体中的5-羟色胺能机制进行了研究。发现垂体中5-羟色胺的浓度存在季节性波动；浓度在冬眠前升高，在冬眠期间下降，并在从冬眠中苏醒时短暂升高。这种模式与5-羟色胺储存在蝙蝠冬眠时变得不活跃的细胞内一致。利用电子显微镜放射自显影以及3H-5-羟色胺的同时光学显微镜放射自显影定位和腺垂体肽的免疫细胞化学定位，对3H-5-羟色胺的摄取进行了研究。发现3H-5-羟色胺的摄取是一个可饱和过程，受氟西汀抑制，但不受去甲肾上腺素模拟或抑制。负责摄取3H-5-羟色胺的细胞含有促黄体生成素免疫反应性，被鉴定为促性腺激素细胞。季节性波动以及特定5-羟色胺摄取机制的存在表明5-羟色胺在腺垂体功能中起作用。
