Carlock L R, Jones N C
J Virol. 1981 Dec;40(3):657-64. doi: 10.1128/JVI.40.3.657-664.1981.
A mutant of adenovirus type 5 containing an octanucleotide insert in region E1a of the viral genome was constructed. The insert was present in only one (13s) of the three overlapping mRNA's synthesized from this region. The insert was within the sequences removed by RNA splicing during the production of the other two nRNA's. The insertion resulted in a shift in the translational reading frame of the 13s mRNA and the probable premature termination of translation. The mutant was defective for viral DNA replication in HeLa cells and the transformation of rat embryo and baby rat kidney cells, indicating that a product encoded by the 13s nRNA is required for these two processes. Other early regions of the genome were expressed in HeLa cells infected by this mutant although in some cases the expression was decreased as compared with wild-type-infected cells.
构建了一种腺病毒5型突变体，其病毒基因组的E1a区域含有一个八核苷酸插入片段。该插入片段仅存在于从该区域合成的三种重叠mRNA中的一种（13s）中。在另外两种mRNA产生过程中通过RNA剪接去除的序列内存在该插入片段。该插入导致13s mRNA的翻译阅读框发生移位，并可能导致翻译过早终止。该突变体在HeLa细胞中进行病毒DNA复制以及转化大鼠胚胎和新生大鼠肾细胞方面存在缺陷，这表明这两个过程需要13s mRNA编码的一种产物。该突变体感染的HeLa细胞中基因组的其他早期区域也有表达，尽管在某些情况下与野生型感染细胞相比表达有所降低。
