腺病毒12型（Ad12）在小鼠L tk-细胞中的E1b启动子被腺病毒E1a区域激活。
The E1b promoter of Ad12 in mouse L tk- cells is activated by adenovirus region E1a.
作者信息
Bos J L, ten Wolde-Kraamwinkel H C
机构信息
Department of Medical Biochemistry, University of Leiden, The Netherlands.
出版信息
EMBO J. 1983;2(1):73-6. doi: 10.1002/j.1460-2075.1983.tb01383.x.
We have investigated the effect of the E1a region of adenovirus on the expression of the E1b transcriptional unit in mouse L tk- cells. To that end we have fused the promoter region of the E1b gene of Ad12 to the coding sequence of the thymidine kinase gene of herpes simplex virus type 1. We found that expression of this fusion gene is completely dependent on the presence of the E1a region. Sequences involved in this regulation were mapped between positions -135 and +11 from the E1b cap site. In addition, we found that the largest E1a protein is essential in this regulation process.
我们研究了腺病毒E1a区域对小鼠L tk-细胞中E1b转录单位表达的影响。为此，我们将Ad12的E1b基因启动子区域与单纯疱疹病毒1型胸苷激酶基因的编码序列融合。我们发现该融合基因的表达完全依赖于E1a区域的存在。参与这种调控的序列定位在E1b帽位点上游-135至+11的位置之间。此外，我们发现最大的E1a蛋白在这一调控过程中至关重要。
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