Holtmann H W, Stein H J
Klin Monbl Augenheilkd. 1978 Dec;173(6):838-42.
20 human donor corneas were placed in a tissue culture medium (NCTC 135 with an additional content of 5% Actovegin resp. Solcoseryl) at +4 degrees C for 5 and 7 days. After that the temperature reversal effect was measured and the corneal endothelial cells were stained. The living cells were counted every 24 hours until no more cells could be seen. It could be shown, that compared with the MK-Medium 24% more living cells could be counted after 5 and 7 days storage in the medium. Possible causes of this improvement, practical consequences with including antibiotic prophylaxis are discussed.
将20个人类供体角膜置于组织培养基(添加5%活心素或素高捷疗的NCTC 135)中,在4摄氏度下保存5天和7天。之后测量温度反转效应并对角膜内皮细胞进行染色。每24小时对活细胞进行计数,直至看不到更多细胞。结果表明,与MK培养基相比,在该培养基中保存5天和7天后可计数的活细胞多24%。讨论了这种改善的可能原因以及包括抗生素预防在内的实际后果。