Holtmann H W, Stein H J
Klin Monbl Augenheilkd. 1976 Mar;168(3):337-41.
20 human donor corneas were placed in a tissue culture medium (TC 199 + 5% Dextran = MK-Medium) at +4 degrees C for 5 and 7 days. After that the temperature reversal effect was measured and the corneal endothelial cells were stained. The living cells were counted every 24 hours until no more cells could be seen. Another group of 20 human donor eyes were placed in to the same medium with a additional content of 5% Actihaemyl resp. Solcoseryl. Same procedure as above. It could be shown that under the influence of the metabolic activator 22.0% and 23.3% more living endothelial cells could be counted and that the total survival time could be prolonged by 1 1/2 days.
将20个人类供体角膜置于组织培养基(TC 199 + 5%葡聚糖=MK培养基)中,在4摄氏度下放置5天和7天。之后测量温度逆转效应并对角膜内皮细胞进行染色。每24小时对活细胞进行计数,直到看不到更多细胞为止。另一组20只人类供体眼被置于相同的培养基中,分别额外添加5%的Actihaemyl或Solcoseryl。操作步骤与上述相同。结果表明,在代谢激活剂的影响下,可以多计数22.0%和23.3%的活内皮细胞,并且总存活时间可以延长1.5天。