Büttner-Ennever J A, Grob P, Akert K, Bizzini B
Neurosci Lett. 1981 Nov 4;26(3):233-8. doi: 10.1016/0304-3940(81)90138-5.
The injection of radioactive [125I]tetanus toxin BIIb fragment into the extraocular eye muscles of monkeys led to strong retrograde labeling of motoneurons. In addition, two patterns of weaker labeling were found: (1) discrete deposits of silver grains associated with cell soma, and (2) diffuse deposits unrelated to cell bodies. The discrete cell soma labeling was found in all areas known to make synaptic contact with the retrogradely filled motoneurons, and is indicative of transsynaptic retrograde transport. The use of tetanus toxin as a transsynaptic retrograde tracer substance is shown here for the first time at the light microscopic level.
将放射性[125I]破伤风毒素BIIb片段注射到猴子的眼外肌中，导致运动神经元出现强烈的逆行标记。此外，还发现了两种较弱的标记模式：（1）与细胞体相关的离散银颗粒沉积物，以及（2）与细胞体无关的弥漫性沉积物。在所有已知与逆行填充的运动神经元形成突触接触的区域都发现了离散的细胞体标记，这表明存在跨突触逆行运输。本文首次在光学显微镜水平展示了将破伤风毒素用作跨突触逆行示踪剂物质的情况。
