Schwab M E, Thoenen H
Brain Res. 1976 Mar 26;105(2):213-27. doi: 10.1016/0006-8993(76)90422-4.
Adult rats were injected intramuscularly (musculus deltoideus) with 125I-labeled tetanus toxin. First signs of tetanic rigidity became visible 12-13 h after injection: death occurred 1-2 h later. Electron microscopic autoradiography of the ventrolateral spinal cord gray matter showed a large part of the radioactivity to be located in motoneurons (perikaryon, axon and dendrites) at 7 and 14 h after injection. A great majority of the rest of the label was found over synaptic terminals, most of them were afferent to motoneurons. The percentage of labeled nerve terminals increased significantly (P less than 0.05) between 7 h and 14 h after injection. Glial elements surrounding the motoneurons were mostly unlabeled. These observations strongly favor the assumption of a transsynaptic migration of tetanus toxin. Dorsal root, dorsal horn and cell bodies of spinal cord interneurons were free of label.
成年大鼠经三角肌肌内注射125I标记的破伤风毒素。注射后12 - 13小时可见破伤风强直的最初迹象，1 - 2小时后死亡。注射后7小时和14小时，对脊髓腹外侧灰质进行电子显微镜放射自显影显示，大部分放射性位于运动神经元（胞体、轴突和树突）。其余大部分标记物位于突触终末，其中大多数是运动神经元的传入终末。注射后7小时至14小时之间，标记神经终末的百分比显著增加（P小于0.05）。运动神经元周围的神经胶质成分大多未被标记。这些观察结果有力地支持了破伤风毒素经突触迁移的假设。脊髓背根、背角和中间神经元的细胞体未被标记。
