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蒙古沙鼠骨髓的细胞学

Cytology of the bone marrow in the Mongolian gerbil.

作者信息

Weeks A M, Glomski C A

出版信息

Lab Anim. 1978 Oct;12(4):195-202. doi: 10.1258/002367778781088486.

DOI:10.1258/002367778781088486
PMID:732260
Abstract

Heterophils were the most numerous cells (39%), followed by the normoblasts (27%) and lymphocytes (8%). Significant differences between males and females were observed in the total number of heterophils (42 and 35% respectively, P less than 0.002), heterophil myelocytes (12 and 10%, P less than 0.01) and the myeloid:erythroid ratio (1.9:1 and 1.4:1, P less than 0.03). Heterophils presented both annular and polymorphous nuclei, which appeared to develop along separate lines. The ring series began with a small nuclear 'vacuole' which increased in size with maturation of the cell. Ring-forms comprised 1/3 of the total heterophil population in the marrow. Eosinophils and basophils also demonstrated annular nucleated forms. Mature heterophils tended to have a scarcity of stainable specific granulation. Eosinophil granules were larger than those of the heterophil, slightly refractile and did not react avidly with eosin. Basophil granules, conversely, reacted strongly with the methylene-blue component of Wright-Giemsa stain and were metachromatic. Normoblasts retained their cytoplasmic basophilia to a greater degree than comparable cells in man.

摘要

异嗜性粒细胞是数量最多的细胞(39%),其次是成红细胞(27%)和淋巴细胞(8%)。在异嗜性粒细胞总数(分别为42%和35%,P<0.002)、异嗜性粒细胞髓细胞(12%和10%,P<0.01)以及髓系与红系比例(1.9:1和1.4:1,P<0.03)方面,观察到雄性和雌性之间存在显著差异。异嗜性粒细胞呈现出环形和多形核,它们似乎沿着不同的途径发育。环形系列始于一个小的核“空泡”,其大小随着细胞成熟而增加。环形细胞占骨髓中异嗜性粒细胞总数的1/3。嗜酸性粒细胞和嗜碱性粒细胞也表现出环形核形态。成熟的异嗜性粒细胞往往缺乏可染色的特异性颗粒。嗜酸性粒细胞的颗粒比异嗜性粒细胞的颗粒大,略有折光性,且与伊红反应不强烈。相反,嗜碱性粒细胞颗粒与瑞氏-吉姆萨染色的亚甲蓝成分反应强烈,且具有异染性。成红细胞比人类的同类细胞更能保持其细胞质嗜碱性。

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