Okada M, Nawa Y, Horii Y, Kitamura T, Arizono N
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
Lab Invest. 1997 Jan;76(1):89-97.
Development of basophilic leukocytes was studied in the Mongolian gerbil, Meriones unguiculatus, after infection with the nematode Nippostrongylus brasiliensis. After infection, peripheral blood basophilia developed and peaked at 2 weeks. In bone marrow sections, numbers of alcian blue+/safranine- basophilic cells were increased. These cells did not bind berberine sulfate and were clearly distinguishable from the bone marrow-resident mast cells, safranine+ and berberine sulfate+. Alcian blue+/safranine- cells were identified by electron microscopy as basophilic myelocytes in various stages of maturation. In the early period of infection, these cells had round-to-oval granules with a homogenous electron-dense matrix, a well-developed Golgi apparatus and rough endoplasmic reticulum, and a nonsegmented nucleus. By enzyme cytochemical analysis, intense peroxidase activity was demonstrated in all of the specific granules as well as in the rough endoplasmic reticulum and Golgi apparatus. Two weeks after infection, the number of bone marrow basophilic cells further increased, forming distinct clusters or islands composed of up to 100 cells each. On electron micrographs, the basophilic cells in these clusters appeared to be late-stage basophilic myelocytes, ie, having an increased number of granules, a less-conspicuous Golgi apparatus and rough endoplasmic reticulum, a horseshoe-shaped-to-lobulated nucleus, and reduced peroxidase activity. Eosinophils and mast cells were rarely found in the basophilic cell clusters. Four weeks after infection, the clusters had disappeared. These results show that gerbil basophilic myelocytes tend to form cell clusters in the bone marrow during their active proliferation. The comparative paucity of other cell lineages in basophilic cell clusters suggests that basophilia is generated from differentiation/proliferation of precommitted basophil progenitors independently from cells of other lineages.
在蒙古沙鼠（Meriones unguiculatus）感染巴西日圆线虫（Nippostrongylus brasiliensis）后，对嗜碱性白细胞的发育进行了研究。感染后，外周血嗜碱性粒细胞增多，并在2周时达到峰值。在骨髓切片中，阿尔辛蓝阳性/番红阴性嗜碱性细胞数量增加。这些细胞不结合硫酸小檗碱，与骨髓中的肥大细胞（番红阳性和硫酸小檗碱阳性）明显不同。通过电子显微镜鉴定，阿尔辛蓝阳性/番红阴性细胞为处于不同成熟阶段的嗜碱性髓细胞。在感染早期，这些细胞具有圆形至椭圆形颗粒，其基质电子密度均匀，高尔基体和粗面内质网发达，细胞核不分叶。通过酶细胞化学分析，在所有特异性颗粒以及粗面内质网和高尔基体中均显示出强烈的过氧化物酶活性。感染后两周，骨髓嗜碱性细胞数量进一步增加，形成由多达100个细胞组成的明显簇或岛。在电子显微镜照片上，这些簇中的嗜碱性细胞似乎是晚期嗜碱性髓细胞，即颗粒数量增加，高尔基体和粗面内质网不那么明显，细胞核呈马蹄形至分叶状，过氧化物酶活性降低。在嗜碱性细胞簇中很少发现嗜酸性粒细胞和肥大细胞。感染后四周，这些簇消失。这些结果表明，沙鼠嗜碱性髓细胞在其活跃增殖期间倾向于在骨髓中形成细胞簇。嗜碱性细胞簇中其他细胞谱系相对较少，这表明嗜碱性粒细胞增多是由预先确定的嗜碱性粒细胞祖细胞的分化/增殖产生的，独立于其他谱系的细胞。
