通过向兔心脏分离的房室结细胞中注入各种钠盐来证明的生电钠泵。

Electrogenic Na pump evidenced by injecting various Na salts into the isolated A-V node cells of rabbit heart.

作者信息

Kurachi Y, Noma A, Irisawa H

出版信息

Pflugers Arch. 1981 Nov;392(1):89-91. doi: 10.1007/BF00584587.

Abstract

Electrogenicity of the Na pump was confirmed by injecting Na salts into a small cluster of A-V node cells. Injection of Na glutamate or Na acetate induced marked hyperpolarization, accompanying with cessation of spontaneous activity. The hyperpolarization exceeded EK in 27 mM K Tyrode solution and was inhibited by 10(-5) M strophanthidin. Injection of NaCl or NaI depolarized the membrane. These data showed that inward-going current carried by the injected anion antagonized the outward-going pump current and thus determined the net effect of the injection.

摘要

通过向一小群房室结细胞中注入钠盐，证实了钠泵的电致性。注入谷氨酸钠或醋酸钠会引起明显的超极化，并伴有自发活动的停止。在含27 mM钾的台氏液中，超极化超过了钾平衡电位（EK），并被10⁻⁵ M毒毛花苷抑制。注入氯化钠或碘化钠会使膜去极化。这些数据表明，注入的阴离子携带的内向电流拮抗了外向泵电流，从而决定了注入的净效应。