Niehaus G D, Dillon B C, Schumacker P T, Saba T M
Proc Soc Exp Biol Med. 1981 Oct;168(1):15-23. doi: 10.3181/00379727-168-41228.
Plasma fibronectin has a high affinity for denatured collagen (gelatin) and exerts an opsonic influence on phagocytosis of test colloids and clearance of tissue debris by macrophages. This study evaluated the effect of in vitro and in vivo interaction of gelatin with plasma on measurable bioassayable opsonic activity and immunoreactive fibronectin. Incubation of human, dog, sheep, and rat plasma with gelatin prior to in vitro assay decreased (P less than 0.05) the ability of plasma to augment particle uptake in the liver slice bioassay. Incubation of plasma with gelatin also decreased the concentration of fibronectin that could be detected by electroimmunoassay. Intravenous infusion of gelatin into rats, dogs, and sheep resulted in an acute depression in both bioassayable and immunoreactive opsonic observations suggest that deficits of opsonic fibronectin as documented in injured patients by bioassay and electroimmunoassay may not be exclusively related to actual depletion of fibronectin from blood but may be, in part, due to binding of fibronectin to blood-borne material post-trauma, i.e., collagenous tissue debris, whose presence in plasma may limit its detection by both assays.
血浆纤维连接蛋白对变性胶原蛋白（明胶）具有高亲和力，并对巨噬细胞吞噬试验胶体和清除组织碎片发挥调理作用。本研究评估了明胶与血浆在体外和体内相互作用对可测量的生物测定调理活性和免疫反应性纤维连接蛋白的影响。在体外试验前将人、狗、绵羊和大鼠血浆与明胶孵育，会降低（P小于0.05）血浆在肝切片生物测定中增强颗粒摄取的能力。血浆与明胶孵育还会降低用电免疫测定法可检测到的纤维连接蛋白浓度。给大鼠、狗和绵羊静脉输注明胶，会导致生物测定和免疫反应性调理观察结果急性下降。这些观察结果表明，通过生物测定和电免疫测定法记录的受伤患者中调理纤维连接蛋白的缺乏可能并非仅与血液中纤维连接蛋白的实际消耗有关，部分原因可能是创伤后纤维连接蛋白与血源物质（即胶原组织碎片）结合，其在血浆中的存在可能会限制两种测定法对其的检测。
