Welsh R
Stain Technol. 1977 Sep;52(5):261-4. doi: 10.3109/10520297709116790.
A procedure is described in which gallein, mordant violet 25, C.I. 45445, is used to demonstrate myelinated nerve fibers in animal brain. Specimens are fixed in 10% neutral buffered formalin and processed in a routine manner. Microsections are stained in an iron gallein solution with subsequent differentiation in 0.25% oxalic acid and 0.1% sodium carbonate solutions that avoid overdifferentiation. Methyl green is used to demonstrate other tissue elements. Myelin is stained deep violet, as are erythrocytes, with neuronal cell bodies and microglia shades of green. The staining procedure requires 30 minutes.
本文描述了一种使用媒染紫25（坚牢黄，C.I. 45445）来显示动物脑内有髓神经纤维的方法。标本用10%中性缓冲福尔马林固定，并按常规方法处理。将薄片在铁坚牢黄溶液中染色，随后在0.25%草酸和0.1%碳酸钠溶液中分化，以避免过度分化。用甲基绿显示其他组织成分。髓磷脂染成深紫色，红细胞也是如此，神经元细胞体和小胶质细胞呈绿色。染色过程需要30分钟。
