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[有髓纤维和脑细胞联合染色的快速方法]

[Rapid method of combined staining of myelinated fibers and brain cells].

作者信息

Viktorov I V

出版信息

Arkh Patol. 1978;40(5):73-6.

PMID:80207
Abstract

A modification of the method of combined staining of myelin fibers and nerve tissue cells (Klüver and Barrera) is offered. Deparaffinised sections are stained for 1--2 hours in 0.01% acid alcohol solution of luxol blue fast, differentiated in 0.1% sodium tetraborate solution, and poststained with cresyl violet fast according to Nissl. The proposed method considerably shortens the time of staining and simplifies the process of differentiation of stained myelin fibers. The prescription for staining of the brain tissue sections silver-impregnated by the method of Holmes with luxol blue fast and cresyl violet is presented.

摘要

提出了一种对髓鞘纤维和神经组织细胞联合染色方法(克吕弗和巴雷拉法)的改良方法。脱蜡切片在0.01% 固蓝快染酸性乙醇溶液中染色1 - 2小时,在0.1% 硼酸钠溶液中分化,然后按照尼斯尔法用固紫快染进行复染。所提出的方法大大缩短了染色时间,并简化了染色髓鞘纤维的分化过程。给出了用福尔摩斯法银浸染的脑组织切片用固蓝快染和固紫快染的染色配方。

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