Bauer E
Res Commun Chem Pathol Pharmacol. 1981 Dec;34(3):547-50.
A new method for collecting tracheobronchial secretions from the anesthesized fowl has been developed. Sulfurdioxide inhalation leads to a highly significant increase of mucus output (wet weight), 320 % of control. Glycoprotein output is increased by only 50 %. Thus glycoprotein concentration is lowered to about 1/3 that of the control group. The carbohydrate pattern of the glycoproteins shows no significant alteration after sulfurdioxide treatment. Isoelectric focusing of te glycoprotein subunits derived from papain digestion shows no difference between the macromolecular pattern of the control and SO2 group.
已开发出一种从麻醉家禽中收集气管支气管分泌物的新方法。吸入二氧化硫会导致黏液分泌量(湿重)显著增加,达到对照组的320%。糖蛋白分泌量仅增加50%。因此,糖蛋白浓度降至对照组的约1/3。二氧化硫处理后,糖蛋白的碳水化合物模式没有显著变化。对木瓜蛋白酶消化产生的糖蛋白亚基进行等电聚焦分析,结果显示对照组和二氧化硫处理组的大分子模式没有差异。