Brinkworth R I, Masters C J
Mech Ageing Dev. 1978 Nov;8(5):299-310. doi: 10.1016/0047-6374(78)90030-1.
The localization of lactate dehydrogenase in the ovaries and reproductive tracts of rats and mice has been studied by a methodology which minimizes loss of this soluble enzyme by diffusion, and allows comment on the subunit composition of the cellular enzyme. The results differ significantly from previous data with conventional methodologies. In particular, the major localization of activity in the present study was identified in interstitial cells, and not the corpora lutea or granulosa cells; and it was noticeable that neither species exhibited massively greater expression of lactate dehydrogenase activity in the oocytes than in adjacent cell types of the reproductive tract. The goblet cells of the Fallopian tube stained intensively for activity of this enzyme. These results have been discussed in relation to the discordant data in the literature, the important role of lactate dehydrogenase in mammalian development, and the evidence for a masking of the activity of this enzyme in oocytes and pre-implantation ova.
通过一种能将这种可溶性酶因扩散造成的损失降至最低的方法，对大鼠和小鼠卵巢及生殖道中乳酸脱氢酶的定位进行了研究，该方法还能对细胞酶的亚基组成进行评述。结果与以往采用传统方法获得的数据有显著差异。特别是，本研究中活性的主要定位在间质细胞，而非黄体或颗粒细胞；值得注意的是，两种物种的卵母细胞中乳酸脱氢酶活性的表达均未大幅高于生殖道相邻细胞类型。输卵管的杯状细胞该酶活性染色强烈。已结合文献中不一致的数据、乳酸脱氢酶在哺乳动物发育中的重要作用以及卵母细胞和植入前卵子中该酶活性被掩盖的证据对这些结果进行了讨论。
