来自中国仓鼠卵巢细胞突变体tsH1回复株中亮氨酰-tRNA合成酶复合物的改造
Reformation of leucyl-tRNA synthetase complexes in revertants from CHO mutant tsH1.
作者信息
Klekamp M, Pahuski E, Hampel A
出版信息
Somatic Cell Genet. 1981 Nov;7(6):725-35. doi: 10.1007/BF01538760.
PMID:7323950
Abstract
A direct correlation was found to exist between increased thermolability of leucyl-tRNA synthetase and loss of the high-molecular-weight enzyme complexes in the CHO cell mutant tsH1 and its revertants. This was shown to occur apart from a differential thermostability between the complexes themselves and is supported by Michaelis constant determinations.
摘要
在CHO细胞突变体tsH1及其回复突变体中，发现亮氨酰-tRNA合成酶的热稳定性增加与高分子量酶复合物的丧失之间存在直接相关性。结果表明，这种情况的发生与复合物本身之间的热稳定性差异无关，并且得到了米氏常数测定结果的支持。
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