用高压液相色谱法测定血浆中的美西律。

Mexiletine in plasma by high pressure liquid chromatography.

作者信息

Kelly R, Christmore D, Smith R, Doshier L, Jacobs S L

出版信息

Ther Drug Monit. 1981;3(3):279-86. doi: 10.1097/00007691-198103000-00009.

DOI:10.1097/00007691-198103000-00009

Abstract

We report here a novel high pressure liquid chromatographic (HPLC) technique for use in therapeutic monitoring of the primary amine antiarrhythmic drug mexiletine in plasma samples. Mexiletine and its structural analogue, the internal standard Ko 768, are extracted with heptane after alkalinization of the sample. The extracts are evaporated to dryness, reconstituted, and injected into a liquid chromatograph equipped with an octadecylsilane reversed phase column using an acetonitrile/acetate buffer mobile phase. Detection is by ultraviolet absorption at 254 nm. The method is linear from 0.2 to 5.0 microgram/ml; the coefficient of variation ranges from 5 to 10% within the therapeutic range. The drug is stable for at least 99 days in samples stored at -20 degrees C. Our HPLC results showed good agreement with a popular gas liquid chromatographic method (14), and there are few incidents of interference from common drugs. The method is convenient for any laboratory equipped with standard liquid chromatographic equipment and is suitable for automation.

摘要

我们在此报告一种新型的高压液相色谱（HPLC）技术，用于血浆样本中伯胺类抗心律失常药物美西律的治疗监测。美西律及其结构类似物内标Ko 768在样品碱化后用庚烷萃取。萃取物蒸发至干，重新溶解，然后注入配备十八烷基硅烷反相柱的液相色谱仪中，使用乙腈/醋酸盐缓冲液流动相。通过在254nm处的紫外吸收进行检测。该方法在0.2至5.0微克/毫升范围内呈线性；在治疗范围内变异系数为5%至10%。该药物在-20℃储存的样品中至少稳定99天。我们的HPLC结果与一种常用的气相色谱法（14）显示出良好的一致性，并且很少有常见药物干扰的情况发生。该方法对任何配备标准液相色谱设备的实验室都很方便，并且适合自动化操作。