Melikiants A G, Matveev V V
Tsitologiia. 1981 Nov;23(11):1242-6.
An attempt was made of quantitative evaluation of the epithelial cell separation in vitro from the descending colon of mice (strains A, BALB/C, CBA, C57Bl and random-bred mice) under unified procedure of the tissue desintegration in norm and under carcinogenesis, depending on the age and strain distinctions. The devised method of forced cell isolation enable us to separate single, differentiated enterocytes, being in the stage of desquamation, from intestinal mucose. The cell separation was shown to decrease with age. The BALC/C mice, with high frequency of 1,2-dimethylhydrasine-induced tumor appearance, were characterized by a more decreased cell separation than C57Bl mice, with low sensitivity to the carcinogen. The cell separation from tumor-free mucosal surface in mice with tumors in colon was also seen decreased, as compared with control animals. The data obtained show that the reported approach of forced cell isolation can be useful for the appraisal of cell loss in the intestine, and suggest that the cell loss is decreasing during cancerogenesis.
在正常和致癌过程中组织崩解的统一程序下，根据年龄和品系差异，尝试对小鼠（A品系、BALB/C品系、CBA品系、C57Bl品系和随机繁殖小鼠）降结肠上皮细胞的体外分离进行定量评估。所设计的强制细胞分离方法使我们能够从肠黏膜中分离出处于脱屑阶段的单个、分化的肠上皮细胞。结果表明，细胞分离随着年龄增长而减少。BALC/C小鼠，1,2 - 二甲基肼诱导肿瘤出现的频率较高，与对致癌物低敏感性的C57Bl小鼠相比，其细胞分离减少得更多。与对照动物相比，结肠有肿瘤的小鼠无肿瘤黏膜表面的细胞分离也减少。所获得的数据表明，所报道的强制细胞分离方法可用于评估肠道中的细胞损失，并表明在癌变过程中细胞损失正在减少。
