Dákay M, Fodor F, Nikodemusz I
Zentralbl Bakteriol Mikrobiol Hyg B. 1981;174(1-2):121-4.
The authors studied the changes in activity of the dehydrogenase enzyme purifier of in the hygiene important bacteria, caused by different concentrations of various detergents with the aid of the triphenyl-tetrazolium chloride test (TTC) (Simmons-Williams test). The cation-active CTAB, in a contentration of only -0.008%, inhibited the enzymatic activity in all bacteria tested, whereas the non-ionic detergents (Praewozell and G 3707), even in high concentrations (5%) produced no inhibiting effect. The anion-active detergents (DBSNa-LSNa) differed in their action on the test bacteria, but Str. faecalis appears to be the most sensitive species, but Micrococcus was also sensitive. In the case of Str. faecalis a 0.03% DBSNa concentration and a 0.008% LSNa concentration was needed to produce the same effect in micrococci. E. coli pathogens proved relatively resistant to anionic detergents; a complete inhibition of the enzymatic activity was brought about by a 1% DBSNa and 0.63% LSNa concentrations. The results suggest that the TTC testing method is suitable for the evaluation of the action of ionic detergents in the organism.
作者借助氯化三苯基四氮唑试验（TTC）（西蒙斯 - 威廉姆斯试验），研究了不同浓度的各种洗涤剂对重要卫生细菌中脱氢酶纯化酶活性的影响。阳离子活性的十六烷基三甲基溴化铵（CTAB），仅0.008%的浓度就抑制了所有测试细菌中的酶活性，而非离子洗涤剂（Praewozell和G 3707），即使在高浓度（5%）下也没有产生抑制作用。阴离子活性洗涤剂（十二烷基苯磺酸钠 - 月桂醇硫酸酯钠）对测试细菌的作用各不相同，但粪肠球菌似乎是最敏感的物种，微球菌也很敏感。对于粪肠球菌，需要0.03%的十二烷基苯磺酸钠浓度和0.008%的月桂醇硫酸酯钠浓度才能在微球菌中产生相同的效果。大肠杆菌病原体对阴离子洗涤剂相对耐药；1%的十二烷基苯磺酸钠和0.63%的月桂醇硫酸酯钠浓度可完全抑制酶活性。结果表明，TTC测试方法适用于评估离子洗涤剂在生物体中的作用。
