离子强度对人红细胞的细胞体积、细胞pH值和细胞缓冲能力的影响。

The effect of ionic strength on cell volume, cell pH and cellular buffer capacity in human red blood cells.

作者信息

Dalmark M

出版信息

Acta Biol Med Ger. 1981;40(6):757-63.

Abstract

The effect of ionic strength on the properties of high molecular weight molecules in human red cells is described. The ionic strength was varied by dialysis of freshly drawn red cells against various KCl solutions in the presence of the ionophore nystatin. Nystatin increased the membrane permeability rather unspecifically towards both monovalent cat- and anions. The membrane permeability towards molecules with an Stokes-Einstein radius of more than 4 A was unaltered. With increasing ionic strength the cellular pH and the cellular volume increased, while the cellular buffer capacity decreased. The variation of the chloride distribution ratio with pH at various KCl concentrations is given in a tabulated form.

摘要

描述了离子强度对人红细胞中高分子量分子性质的影响。通过在离子载体制霉菌素存在的情况下，将新鲜采集的红细胞与各种氯化钾溶液进行透析来改变离子强度。制霉菌素对单价阳离子和阴离子的膜通透性增加较为非特异性。对斯托克斯 - 爱因斯坦半径大于4埃的分子的膜通透性未改变。随着离子强度增加，细胞pH值和细胞体积增加，而细胞缓冲能力下降。以表格形式给出了在不同氯化钾浓度下氯化物分布比随pH值的变化情况。