Drake K L, Eckstein E C
Artif Organs. 1981 Nov;5(4):363-71. doi: 10.1111/j.1525-1594.1981.tb04018.x.
Experimental methods and apparatus were developed to measure platelet concentration in an ultrafiltering hollow fiber with blood and ultrafiltrative flow rates that are characteristic of current hemofiltration practice. Values are presented for the adjusted local platelet concentration, which allows for the expected increase due to loss of ultrafiltrate volume. Platelet concentrations in fiber samples are of interest since ultra-filtration can create platelet polarization in a fashion similar to the concentration polarization that occurs for proteins. For many combinations of inlet flow rate, ultrafiltrative flow rate, and fiber dimension, there are significant elevations in adjusted fiber platelet concentration. The adjusted platelet concentrations of the outflow samples are nominally equal to those of the feed (reservoir) samples; thus, reaction and adhesion of platelets is not a significant source of fiber concentration. Experiments with fibers of different diameters indicate that, as expected in concentration polarization, the excess platelets are located near the wall. Data for the fiber platelet concentration depend in a complex fashion upon the inlet wall shear rate and ultrafiltrative velocity. For certain fixed ultrafiltrative velocities, a maximum fiber platelet concentration occurs at an intermediate inlet wall shear rate. The data are qualitatively explained by an extension of a published model involving diffusive, ultrafiltrative, and wall-induced fluxes of red cells and platelets.
开发了实验方法和装置，以测量具有血液和超滤流速的超滤中空纤维中的血小板浓度，这些流速是当前血液滤过实践的特征。给出了调整后的局部血小板浓度值，该值考虑了由于超滤体积损失导致的预期增加。纤维样品中的血小板浓度受到关注，因为超滤可以以类似于蛋白质发生的浓度极化的方式产生血小板极化。对于许多入口流速、超滤流速和纤维尺寸的组合，调整后的纤维血小板浓度有显著升高。流出样品的调整后血小板浓度名义上等于进料（储液器）样品的浓度；因此，血小板的反应和粘附不是纤维浓度的重要来源。不同直径纤维的实验表明，正如浓度极化中预期的那样，过量的血小板位于壁附近。纤维血小板浓度的数据以复杂的方式取决于入口壁剪切速率和超滤速度。对于某些固定的超滤速度，在中间入口壁剪切速率下会出现最大纤维血小板浓度。通过扩展已发表的涉及红细胞和血小板的扩散、超滤和壁诱导通量的模型，对数据进行了定性解释。
