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无内毒素人促红细胞生成素的生物学特性

The biological properties of endotoxin-free human erythropoietin.

作者信息

Weiss T L, Goldwasser E

出版信息

Biochem J. 1981 Jul 15;198(1):17-21. doi: 10.1042/bj1980017.

DOI:10.1042/bj1980017
PMID:7325992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163205/
Abstract

The biological effects formerly attributed to erythropoietin were generally observed using impure preparations of the hormone containing, among the impurities, bacterial endotoxin, a contaminant that has recently been shown to affect the haemopoietic system. Erythropoietin, purified to apparent homogeneity and freed of trace contamination by endotoxin (1) promotes erythroid burst formation from mouse marrow cells in semi-solid culture medium, (2) stimulates haemoglobin synthesis in rat marrow cell cultures, (3) stimulates transcription in mouse and rat bone marrow cell cultures and (4) causes the formation of the cytoplasmic mediator protein in rat marrow cells, which enhances transcription in isolated nuclei.

摘要

以前归因于促红细胞生成素的生物学效应，通常是使用该激素的不纯制剂观察到的，这些制剂的杂质中含有细菌内毒素，最近已证明这种污染物会影响造血系统。将促红细胞生成素纯化至表观均一且无内毒素微量污染后，（1）在半固体培养基中可促进小鼠骨髓细胞形成红系爆式集落，（2）刺激大鼠骨髓细胞培养物中的血红蛋白合成，（3）刺激小鼠和大鼠骨髓细胞培养物中的转录，（4）促使大鼠骨髓细胞中形成细胞质介质蛋白，该蛋白可增强分离细胞核中的转录。

相似文献

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The biological properties of endotoxin-free human erythropoietin.无内毒素人促红细胞生成素的生物学特性
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In vivo and in vitro effect of bacterial endotoxin on erythroid precursors (CFU-E and ERC) in the bone marrow of mice.细菌内毒素对小鼠骨髓中红系前体细胞（CFU-E和ERC）的体内和体外作用。
J Lab Clin Med. 1977 Feb;89(2):278-84.
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Stem cell migration induced by erythropoietin or haemolytic anaemia: the effects of actinomycin and endotoxin contamination of erythropoietin preparations.促红细胞生成素或溶血性贫血诱导的干细胞迁移：促红细胞生成素制剂中放线菌素和内毒素污染的影响。
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On the mechanism of erythropoientin-induced differentiation. X. The effect of thymidine suicide on erythropoietin-responsive cells.论促红细胞生成素诱导分化的机制。十、胸苷自杀对促红细胞生成素反应性细胞的影响。
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Erythropoietin action in rat marrow cell cultures in complete absence of dna synthesis. II. Long term effects on macromolecular synthesis.在完全缺乏DNA合成的大鼠骨髓细胞培养物中促红细胞生成素的作用。II. 对大分子合成的长期影响。
Biochem Biophys Res Commun. 1976 Mar 22;69(2):489-98. doi: 10.1016/0006-291x(76)90548-9.

本文引用的文献

1
THE EFFECT OF ERYTHROPOIETIN UPON HEME SYNTHESIS BY MARROW CELLS IN VITRO.促红细胞生成素对体外培养的骨髓细胞血红素合成的影响。
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A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
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Effect of erythropoietin upon nucleic acid synthesis in erythropoietic tissue.促红细胞生成素对造血组织中核酸合成的影响。
J Lab Clin Med. 1966 Jun;67(6):937-46.
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On the mechanism of erythropoietin-induced differentiation. V. Characterization of the ribonucleic acid formed as a result of erythropoietin action.论促红细胞生成素诱导分化的机制。V. 促红细胞生成素作用所形成的核糖核酸的特性
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Protein synthesis: its control in erythropoiesis.蛋白质合成：其在红细胞生成中的调控
Science. 1972 Mar 3;175(4025):955-61. doi: 10.1126/science.175.4025.955.
6
Erythropoietin effects on fetal mouse erythroid cells. II. Nucleic acid synthesis and the erythropoietin-sensitive cell.促红细胞生成素对胎鼠红系细胞的作用。II. 核酸合成与促红细胞生成素敏感细胞
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On the mechanism of erythropoietin-induced differentiation. XII. A cytoplasmic protein mediating induced nuclear RNA synthesis.促红细胞生成素诱导分化的机制。十二、一种介导诱导性核RNA合成的细胞质蛋白。
Dev Biol. 1973 Oct;34(2):246-54. doi: 10.1016/0012-1606(73)90353-9.
8
Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria.内毒素的皮克级灵敏检测法：革兰氏阴性菌纯化脂多糖和脂质A诱导鲎血细胞溶解物凝胶化。
Biochim Biophys Acta. 1972 Jan 28;261(1):284-9. doi: 10.1016/0304-4165(72)90340-6.
9
Synthesis of macromolecules during induction of haemoglobin synthesis by erythropoietin.促红细胞生成素诱导血红蛋白合成过程中大分子的合成
J Mol Biol. 1969 May 28;42(1):31-41. doi: 10.1016/0022-2836(69)90484-7.
10
Sequential activation of splenic nuclear RNA polymerases by erythropoietin.促红细胞生成素对脾细胞核RNA聚合酶的顺序激活作用。
J Clin Invest. 1976 Jan;57(1):20-6. doi: 10.1172/JCI108260.

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