使用辣根过氧化物酶-凝集素偶联物来监测从分离细胞中获得的质膜制剂的纯度。
The use of horseradish peroxidase-lectin conjugates to monitor the purity of plasma-membrane preparations obtained from isolated cells.
作者信息
Banga J P, Penfold P, Roitt I M
出版信息
Biochem J. 1981 Aug 15;198(2):421-3. doi: 10.1042/bj1980421.
Abstract
The plasma membranes of viable murine EL4 tumour cells were labelled with horseradish peroxidase-conjugated wheat-germ agglutinin. After disruption of the labelled intact cells, plasma-membrane purification could be monitored by ultrastructural examination of the various fractions for positive reaction product on the membrane vesicles.
摘要
用辣根过氧化物酶偶联的麦胚凝集素标记存活的小鼠EL4肿瘤细胞的质膜。在破坏标记的完整细胞后，可通过对各个组分进行超微结构检查，以检测膜泡上的阳性反应产物来监测质膜的纯化情况。
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