重封猪淋巴细胞质膜囊泡的制备。
Preparation of resealed pig lymphocyte plasma-membrane vesicles.
作者信息
Johnstone A P, Crumpton M J
出版信息
Biochem J. 1980 Jul 15;190(1):45-50. doi: 10.1042/bj1900045.
Abstract
Pig lymphocyte plasma-membrane vesicles that are impermeable to large molecules were prepared by means of centrifugation on a continuous dextran gradient. They were isolated in a yield of 60--80% of the total plasma membrane recovered, and were characterized as being impermeable to macromolecules by their exclusion of inulin (mol.wt. 5200) and Dextran T10 (mol.wt. 10 000).
摘要
通过在连续葡聚糖梯度上离心制备了对大分子不可渗透的猪淋巴细胞质膜囊泡。它们的分离产率为回收的总质膜的60%-80%，通过排除菊粉（分子量5200）和葡聚糖T10（分子量10000）表明其对大分子不可渗透。
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