[乙醇及其生物转化产物对大鼠植入后胚胎培养物的胚胎毒性作用]
[Embryotoxic effect of ethanol and its biotransformation products in cultures of postimplantation rat embryos].
作者信息
Popov V B, Vaisman B L, Puchkov V F, Ignat'eva T V
出版信息
Biull Eksp Biol Med. 1981 Dec;92(12):725-8.
A study was made of the effect of ethanol (6.5-108 mM) and its immediate metabolite, acetaldehyde (4.5 microM-45 mM) on 9.5-day-old rat embryos cultivated in vitro. Biotransformation of the nontoxic concentration of ethanol (17 mM) to teratogenic products was made by addition to the culture medium of a soluble fraction of the rat liver (0.2-0.55 mg protein/ml), NAD (0.25 mM) and sodium pyruvate (2.3 mM). Malformations obtained after biotransformation of ethanol were similar to those obtained after direct action of acetaldehyde. The roles of acetaldehyde and ethanol in the etiology of fetal alcoholic injury are discussed.
研究了乙醇（6.5 - 108 mM）及其直接代谢产物乙醛（4.5 microM - 45 mM）对体外培养的9.5日龄大鼠胚胎的影响。通过向培养基中添加大鼠肝脏的可溶性部分（0.2 - 0.55 mg蛋白质/ml）、NAD（0.25 mM）和丙酮酸钠（2.3 mM），将无毒浓度的乙醇（17 mM）生物转化为致畸产物。乙醇生物转化后获得的畸形与乙醛直接作用后获得的畸形相似。讨论了乙醛和乙醇在胎儿酒精性损伤病因学中的作用。
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