Potter V R
Carcinogenesis. 1981;2(12):1375-9. doi: 10.1093/carcin/2.12.1375.
A new protocol for carcinogenesis in rat liver is described in order that confirmatory experiments might be undertaken concurrently. The basic protocol, designated IPI (initiator + promoter + initiator), is presented in several alternative forms including the possible use of X-irradiation as the initiator. The rationale is discussed in terms of the two-hit somatic mutation theory of Armitage and Doll, with an initial hit produced by the first dose of initiator and expansion of single cells to sizable clones by promotion thereby increasing the probability of a second hit by the second dose of initiator. The question of relevant mutations is taken up and it is proposed that genes for chalones and for chalone receptors are logical targets for consideration in a two-mutation sequence.
本文描述了一种新的大鼠肝癌发生实验方案，以便能同时进行验证性实验。基本方案被命名为IPI（启动剂+促进剂+启动剂），有几种不同形式，包括可能使用X射线作为启动剂。依据阿米蒂奇和多尔的双打击体细胞突变理论对该原理进行了讨论，首次剂量的启动剂产生初始打击，促进剂使单个细胞扩增为相当大的克隆，从而增加了第二次剂量启动剂产生第二次打击的可能性。文中讨论了相关突变的问题，并提出抑素和抑素受体的基因是双突变序列中值得考虑的合理靶点。
