Hellmann A, Goldman J M, Spry C J
Clin Lab Haematol. 1981;3(3):205-13. doi: 10.1111/j.1365-2257.1981.tb01334.x.
Colony formation by granulocyte/macrophage and eosinophilic progenitor cells in the blood and/or bone marrow of four patients with "hypereosinophilic syndrome' (HES) was studied in agar culture. Colony-stimulating activity (CSA) was derived from leucocyte feeder layers (LFL) or from medium conditioned by phytohaemagglutinin-stimulated lymphocytes (LCM). The total numbers of colonies in the patients' blood and marrow were normal. When patients' marrow cells were cultured over LFL, the proportion of colonies that were eosinophilic was greater than normal (mean 21% vs 6%, P less 0.001) and this difference was accentuated when CSA was derived from LCM (mean 53% vs 15%, P less than 0.001). LCM derived from normal subjects and LCM prepared from HES patients had similar effects. In the blood the total number of colonies and the proportion of eosinophilic colonies were similar in patients and controls. The overall pattern of colony formation HES differed distinctly from that observed in a patient with eosinophilic leukaemia reported previously. We conclude that the proportion of eosinophil-committed progenitor cells in the marrow of patients with HES is increased, but we have failed to demonstrate a role for the patient's own lymphocytes in augmenting eosinophil production.
对4例“高嗜酸性粒细胞综合征”（HES）患者血液和/或骨髓中的粒细胞/巨噬细胞及嗜酸性祖细胞的集落形成进行了琼脂培养研究。集落刺激活性（CSA）来源于白细胞饲养层（LFL）或植物血凝素刺激的淋巴细胞条件培养液（LCM）。患者血液和骨髓中的集落总数正常。当患者骨髓细胞在LFL上培养时，嗜酸性集落的比例高于正常（平均21%对6%，P<0.001），当CSA来源于LCM时，这种差异更加明显（平均53%对15%，P<0.001）。来自正常受试者的LCM和由HES患者制备的LCM具有相似的作用。在血液中，患者和对照组的集落总数及嗜酸性集落比例相似。HES患者的集落形成总体模式与先前报道的1例嗜酸性粒细胞白血病患者明显不同。我们得出结论，HES患者骨髓中嗜酸性粒细胞定向祖细胞的比例增加，但我们未能证明患者自身淋巴细胞在增加嗜酸性粒细胞生成中起作用。
