Bjornson B H, Harley J B, André-Schwartz J, Fauci A S, Desforges J F
Blood. 1982 Sep;60(3):721-6.
Myeloid progenitor cell cultures (CFU-C) were established in a double-layer agar system with peripheral blood mononuclear cells from 13 patients with the hypereosinophilic syndrome (HES). Normal controls produced 49% +/- 3.5% eosinophil colonies; results in 7 of the 13 HES patients were within the normal range, while in 5, the proportion of eosinophil colonies was greater than 3 standard deviations above the normal mean, and in 1 patient there was a low proportion of eosinophil colonies. The production of an increased proportion of eosinophil colonies correlated with more aggressive disease. Experiments in which normal progenitor cells were cultured over feeder layers of mononuclear cells demonstrated that cells of 3 of the 5 patients had an excess production of eosinophil colony-stimulating activity. When HES patients progenitor cells were cultured over normal feeder layers, 2 of the 5 patient samples continued to produce an increased proportion of eosinophil colonies, suggesting that these patients have an excess proportion of progenitor cells committed to eosinophil differentiation. Thus, the results demonstrated heterogeneity of growth characteristics for the HES patients. None, however, had the colony growth characteristic of acute or chronic myelogenous leukemia.
采用双层琼脂系统，以13例高嗜酸性粒细胞综合征（HES）患者的外周血单个核细胞建立髓系祖细胞培养体系（CFU - C）。正常对照产生49%±3.5%的嗜酸性粒细胞集落；13例HES患者中，7例结果在正常范围内，5例嗜酸性粒细胞集落比例高于正常均值3个标准差以上，1例患者嗜酸性粒细胞集落比例较低。嗜酸性粒细胞集落比例增加与更具侵袭性的疾病相关。将正常祖细胞在单个核细胞饲养层上培养的实验表明，5例患者中有3例的细胞产生过量的嗜酸性粒细胞集落刺激活性。当将HES患者的祖细胞在正常饲养层上培养时，5例患者样本中有2例继续产生比例增加的嗜酸性粒细胞集落，提示这些患者中致力于嗜酸性粒细胞分化的祖祖祖祖细胞比例过高。因此，结果显示HES患者的生长特征具有异质性。然而，无一例具有急性或慢性髓性白血病的集落生长特征。
