Datta S, Datta S C
Experientia. 1981 Dec 15;37(12):1260-1. doi: 10.1007/BF01948347.
Buffalo alpha S1-casein was treated with carboxypeptidase-A to determine the C-terminal amino acid residue, which was - leucine-tryptophan. The Sakaguchi reagent was used to confirm arginine as the N-terminal amino acid. The colored p-phenylazophenyl thiohydantoins of tryptophan and arginine released from C- and N-terminals respectively were quantitatively estimated and from this molecular weights of buffalo alpha S1 casein were calculated and found to be 26,300 and 26,100 respectively.
用羧肽酶 A 处理水牛α-S1-酪蛋白以确定 C 末端氨基酸残基，其为亮氨酸-色氨酸。使用坂口试剂确认精氨酸为 N 末端氨基酸。分别从 C 末端和 N 末端释放的色氨酸和精氨酸的有色对苯基偶氮苯硫代乙内酰脲进行定量测定，并据此计算出水牛α-S1-酪蛋白的分子量，分别为 26300 和 26100。
