Matsuzaki S
Nihon Naibunpi Gakkai Zasshi. 1981 Oct 20;57(10):1554-66. doi: 10.1507/endocrine1927.57.10_1554.
Changes in polyamine contents and in the activity of polyamine biosynthetic enzymes were studied in the rat thyroid. Both exogenous and endogenous TSH promptly stimulated the activity of thyroid ornithine decarboxylase (ODC) and S-adenosyl-L-methionine decarboxylase. Treatment of the animals with antithyroid drugs resulted in an increase in circulating TSH level, the activities of the enzymes and thyroidal contents of putrescine, spermidine and spermine. Theophylline, which stimulated methylthiouracil (MTU)-induced goitrogenesis without increasing circulating TSH level, increased the thyroid ODC activity. In contrast, treatment with excess iodine (KI) which induced thyroid involution without affecting circulating levels of TSH, T4 and T3, resulted in a rapid reduction in the thyroid ODC activity. The MTU-induced goiter involuted after MTU withdrawal and the thyroid ODC and polyamine contents decreased within a few days after its withdrawal. The activity of thyroid arginase was increased by MTU and decreased by T4 injection. The enzyme may have some regulatory role in polyamine biosynthesis by controlling ornithine supply. Polyamine levels were elevated in both papillary and follicular adenocarcinomas of the human thyroid. The results suggest that the measurement of thyroid polyamines may be useful for the diagnosis of carcinomas.
对大鼠甲状腺中的多胺含量及多胺生物合成酶活性的变化进行了研究。外源性和内源性促甲状腺激素（TSH）均能迅速刺激甲状腺鸟氨酸脱羧酶（ODC）和S-腺苷-L-甲硫氨酸脱羧酶的活性。用抗甲状腺药物处理动物会导致循环TSH水平升高、这些酶的活性以及甲状腺中腐胺、亚精胺和精胺的含量增加。茶碱能刺激甲硫氧嘧啶（MTU）诱导的甲状腺肿形成，且不增加循环TSH水平，它可提高甲状腺ODC活性。相反，用过量碘（KI）处理可导致甲状腺萎缩，而不影响TSH、T4和T3的循环水平，同时会使甲状腺ODC活性迅速降低。MTU停药后，MTU诱导的甲状腺肿会消退，停药后几天内甲状腺ODC和多胺含量会降低。MTU可提高甲状腺精氨酸酶的活性，而注射T4则使其活性降低。该酶可能通过控制鸟氨酸供应在多胺生物合成中发挥某种调节作用。人甲状腺的乳头状癌和滤泡状腺癌中的多胺水平均升高。结果表明，检测甲状腺多胺可能有助于癌症的诊断。
