Sieber F, Meagher R C, Spivak J L
Differentiation. 1981;19(1):65-7. doi: 10.1111/j.1432-0436.1981.tb01129.x.
In vivo and in vitro clonal assays of immature mouse blood cells showed that different populations of hematopoietic progenitor cells differ considerably with respect to their sensitivity to photodynamic damages caused by the fluorescent dye Merocyanine 540. Late erythroid progenitors were the most sensitive cells followed in order of decreasing sensitivity by pluripotent stem cells, early erythroid progenitors, and granulocyte/macrophage progenitors. Only about 2%-4% of all nucleated marrow cells were stained with Merocyanine 540 which correlated well with current frequency estimates of progenitor cells in mouse bone marrow. Our findings indicate that the expression of Merocyanine binding sites is developmentally regulated and might, therefore, provide a useful molecular marker for blood cell differentiation and a basis for an effective purification of hematopoietic progenitor cells.
对未成熟小鼠血细胞进行的体内和体外克隆分析表明，不同群体的造血祖细胞对荧光染料部花青540引起的光动力损伤的敏感性存在显著差异。晚期红系祖细胞是最敏感的细胞，其次是多能干细胞、早期红系祖细胞和粒细胞/巨噬细胞祖细胞，敏感性依次降低。所有有核骨髓细胞中只有约2%-4%被部花青540染色，这与目前对小鼠骨髓中祖细胞频率的估计非常吻合。我们的研究结果表明，部花青结合位点的表达受发育调控，因此可能为血细胞分化提供一个有用的分子标记，并为有效纯化造血祖细胞提供基础。
