Nomoto C, Suemasu K, Higashi Y, Takeuchi O, Shisa H, Tsuchiya S, Hozumi M, Yoshida S
Gan. 1981 Oct;72(5):783-9.
A new human cell line, Br-NHF-1, was established from the pleural effusion of a patient with advanced mammary carcinoma. The cells survived 52 subcultivations during more than 33 months. The cells possess epithelial features, showing rosettes, acinar formation and domes in or among the compact colonies. The modal chromosome number is 51 with some marker chromosomes. Xenografted tumors retained a similar histology to the original tumor. No estrogen-binding protein was detectable in the culture, but there was a significant amount of basic fetoprotein. Production of casein in the cells was detected by immuno-fluorescence testing.
一种新的人类细胞系Br-NHF-1，是从一名晚期乳腺癌患者的胸腔积液中建立的。这些细胞在超过33个月的时间里存活了52代。细胞具有上皮特征，在紧密的集落内部或之间形成玫瑰花结、腺泡结构和穹顶。众数染色体数为51，并伴有一些标记染色体。异种移植肿瘤保留了与原始肿瘤相似的组织学特征。在培养物中未检测到雌激素结合蛋白，但存在大量碱性甲胎蛋白。通过免疫荧光检测在细胞中检测到了酪蛋白的产生。
