Grimmelikhuijzen C J, Dockray G J, Yanaihara N
Histochemistry. 1981;73(2):171-80. doi: 10.1007/BF00493017.
With immunocytochemical methods, nerve cells have been detected in Hydra attenuata containing bombesin-like immunoreactivity. These nerve cells are located in ectoderm of all body regions of the animal and are especially abundant in basal disk and tentacles. Radioimmunoassay of extracts of hydra demonstrated at least 0.2 pmol/g wet weight of bombesin-like immunoreactivity. The immunoreactive material elutes from Sephadex G-50 in a similar position to synthetic bombesin. The data show that bombesin-like peptides are among the phylogenetically oldest neuropeptides found so far.
运用免疫细胞化学方法，在细螅中检测到含有蛙皮素样免疫反应性的神经细胞。这些神经细胞位于该动物身体所有部位的外胚层，在基盘和触手中尤为丰富。对细螅提取物进行放射免疫测定表明，蛙皮素样免疫反应性至少为0.2皮摩尔/克湿重。免疫反应性物质从葡聚糖凝胶G - 50上洗脱的位置与合成蛙皮素相似。数据表明，蛙皮素样肽是迄今为止发现的系统发育上最古老的神经肽之一。
