Moody T W, Russell E K, O'Donohue T L, Linden C D, Gazdar A F
Life Sci. 1983 Jan 31;32(5):487-93. doi: 10.1016/0024-3205(83)90142-x.
High intracellular levels of BN-like peptides are present in tumors and cell lines of small cell carcinoma of the lung (SCCL) as well as the putative precursor cells of this tumor, the pulmonary endocrine cell. In cell line NCI-H209 the density of bombesin-like peptides was 8.9 +/- 1.1 pmol/mg total protein. Gel filtration chromatography of an extract of these cells revealed one major peak of immunoreactivity which coeluted with synthetic bombesin (1620 daltons). Also, high pressure liquid chromatography revealed one major peak of immunoreactivity was present which eluted before synthetic peptide. Therefore, SCCL bombesin-like peptides may be of similar size but are more hydrophilic than synthetic peptide. Cells maintained in culture continuously release bombesin-like peptides into the growth medium. Also, high concentrations of K+ stimulated the secretion of immunoreactive bombesin from cell lines in a Ca++-dependent manner. These SCCL bombesin-like peptides may function as important regulatory agents in the malignant lung.
在肺小细胞癌（SCCL）的肿瘤组织和细胞系以及该肿瘤的假定前体细胞——肺内分泌细胞中，存在高水平的蛙皮素样肽。在细胞系NCI-H209中，蛙皮素样肽的密度为8.9±1.1 pmol/mg总蛋白。对这些细胞提取物进行凝胶过滤层析，显示出一个主要的免疫反应峰，它与合成蛙皮素（1620道尔顿）共洗脱。此外，高压液相色谱显示存在一个主要的免疫反应峰，其在合成肽之前洗脱。因此，SCCL蛙皮素样肽可能大小相似，但比合成肽更具亲水性。培养中的细胞持续将蛙皮素样肽释放到生长培养基中。此外，高浓度的K+以Ca++依赖的方式刺激细胞系分泌免疫反应性蛙皮素。这些SCCL蛙皮素样肽可能在恶性肺组织中作为重要的调节因子发挥作用。
