Kintaka K, Haibara K, Asai M, Imada A
J Antibiot (Tokyo). 1981 Sep;34(9):1081-9. doi: 10.7164/antibiotics.34.1081.
A novel beta-lactam antibiotic, isosulfazecin (iSZ), was found to be produced by an acidophilic pseudomonad, Pseudomonas mesoacidophila sp. nov. iSZ was produced in parallel with bacterial growth in nutrient broth containing glycerol and sodium thiosulfate under aerated conditions. iSZ was isolated by chromatography on activated charcoal and anion-exchangers and crystallized from 70% aqueous methanol. The molecular formula was determined to be C12H20N4O9S from physiochemical data. The IR and NMR spectra suggested that iSZ has a beta-lactam ring, methoxyl and sulfonate groups. On acid hydrolysis, it gave L-alanine and D-glutamic acid. iSZ is an epimeric isomer of sulfazecin. iSZ was weakly active against Gram-positive and -negative bacteria, and was strongly active against mutants hypersensitive to beta-lactam antibiotics.
一种新型β-内酰胺抗生素异硫杂环菌素（iSZ），被发现由嗜酸性假单胞菌——嗜中酸性假单胞菌新种产生。在通气条件下，iSZ在含有甘油和硫代硫酸钠的营养肉汤中与细菌生长同时产生。iSZ通过在活性炭和阴离子交换剂上的色谱法分离，并从70%的甲醇水溶液中结晶。根据理化数据确定其分子式为C12H20N4O9S。红外光谱和核磁共振光谱表明iSZ具有β-内酰胺环、甲氧基和磺酸根基团。经酸水解后，它产生L-丙氨酸和D-谷氨酸。iSZ是硫杂环菌素的差向异构体。iSZ对革兰氏阳性菌和阴性菌活性较弱，但对β-内酰胺抗生素超敏突变体具有较强活性。
