使用硫酸铵、聚乙二醇或中空纤维超滤法浓缩人呼吸道合胞病毒。

Concentration of human respiratory syncytial virus using ammonium sulfate, polyethylene glycol or hollow fiber ultrafiltration.

作者信息

Trépanier P, Payment P, Trudel M

出版信息

J Virol Methods. 1981 Nov;3(4):201-11. doi: 10.1016/0166-0934(81)90071-9.

Abstract

Human respiratory syncytial virus was concentrated by polyethylene glycol or ammonium sulfate precipitation as well as by hollow ultrafiltration. Recoveries obtained were respectively 49.4%, 47.7%, and 75.2%; however, further analysis of these results by resuspension experiments showed that all the infectivity could be recovered from the different concentrates. The protein content of polyethylene glycol concentrates was much lower than those of ammonium sulfate or hollow fiber ultrafiltration. Electron microscopy revealed that the morphological integrity of virus particles was unaffected by the concentration methods used. Purified virus was obtained when polyethylene glycol concentrates were centrifuged through two successive density gradients.

摘要

人呼吸道合胞病毒通过聚乙二醇或硫酸铵沉淀以及中空纤维超滤进行浓缩。获得的回收率分别为49.4%、47.7%和75.2%；然而，通过重悬实验对这些结果进行进一步分析表明，所有传染性均可从不同的浓缩物中回收。聚乙二醇浓缩物的蛋白质含量远低于硫酸铵或中空纤维超滤浓缩物。电子显微镜显示，病毒颗粒的形态完整性不受所用浓缩方法的影响。当聚乙二醇浓缩物通过两个连续的密度梯度离心时，可获得纯化的病毒。