用于鉴定人呼吸道合胞病毒多肽的改良免疫沉淀程序。

Modified immunoprecipitation procedure for the identification of human respiratory syncytial virus polypeptides.

作者信息

Trépanier P, Payment P, Trudel M

出版信息

J Virol Methods. 1983 Sep;7(3):149-54. doi: 10.1016/0166-0934(83)90004-6.

Abstract

When analysed by polyacrylamide gel electrophoresis, human respiratory syncytial virus harvested after a one step growth cycle and purified through a continuous sucrose density gradient was shown to be composed of nine structural proteins of 90, 68, 49, 42, 34, 28, 25, 19 an 13 kd. The 90, 49 and 19 kd polypeptides were identified as glycopolypeptides by glucosamine incorporation. A modified immunoprecipitation procedure confirmed the viral specificity of the 49, 42, 28, 25 and 19 kd polypeptides.

摘要

通过聚丙烯酰胺凝胶电泳分析，经一步生长周期收获并通过连续蔗糖密度梯度纯化的人呼吸道合胞病毒显示由90、68、49、42、34、28、25、19和13kd的九种结构蛋白组成。通过氨基葡萄糖掺入将90、49和19kd多肽鉴定为糖多肽。一种改良的免疫沉淀程序证实了49、42、28、25和19kd多肽的病毒特异性。