Njogu R M, Nyindo M
J Parasitol. 1981 Dec;67(6):847-51.
We have studied metabolism of glucose by infective forms of Trypanosoma brucei brucei isolated from tsetse fly salivary glands and grown in continuous culture for more than 700 days. The end products of glucose metabolism under aerobic conditions were found to be pyruvate and glycerol in the ratio 3:1. this changed to equimolar formation of pyruvate and glycerol when glucose was metabolized under aerobic conditions in the presence of 1.5 mM salicylhydroxamic acid (SHAM), a situation analogous to anaerobiosis. The respiration of the parasites was insensitive to cyanide inhibition but SHAM inhibited it completely. In the presence of SHAM, formation of pyruvate and glycerol from glucose metabolism was inhibited by 60% and 45% respectively. These findings indicate that the infective trypanosomes grown in vitro for about 2 yr have a peculiar glycolytic pathway which can be exploited to elucidate the mechanism of energy production of bloodstream form trypanosomes during anaerobic glycolysis.
我们研究了从采采蝇唾液腺分离并在连续培养中生长超过700天的布氏布氏锥虫感染性形态的葡萄糖代谢。在有氧条件下，葡萄糖代谢的终产物被发现是丙酮酸和甘油，其比例为3:1。当在1.5 mM水杨羟肟酸（SHAM）存在下于有氧条件下代谢葡萄糖时，丙酮酸和甘油以等摩尔形成，这种情况类似于无氧状态。寄生虫的呼吸对氰化物抑制不敏感，但SHAM完全抑制了它。在SHAM存在下，葡萄糖代谢产生丙酮酸和甘油分别被抑制60%和45%。这些发现表明，体外培养约2年的感染性锥虫具有独特的糖酵解途径，可用于阐明血流型锥虫在无氧糖酵解过程中的能量产生机制。
