Ito M, Yamahata K, Yagi N, Sakamoto M, Sekikawa H, kadowaki J, Ohnishi M, Itoh H, Takada M
J Pharmacobiodyn. 1981 Nov;4(11):901-6. doi: 10.1248/bpb1978.4.901.
Biochemical and biophysical analyses were made to study the properties of serum albumin from nephrotic patients of two types, remissive stage and relapsed state, and the results were compared with those in normal subjects. Two different ion exchange columns were prepared to isolate and purify each albumin sample and the materials eluted through the two columns were identified. The nephrotic elution materials, especially in the state of relapse, were of some difference from those of normal subjects. alpha-Helix contents estimated by circular dichroism spectra were found to be closer to normal albumin in remissive state than in the state of relapse. The binding of fluorescent probe, 1-anilino-8-naphthalene sulfonate, to albumin was examined by calculating the binding parameter, the dissociation constant and number of binding sites. The binding parameters were closer to those of normal albumin in remissive stage than the state of relapse.
进行了生化和生物物理分析，以研究两种类型的肾病患者（缓解期和复发期）血清白蛋白的特性，并将结果与正常受试者的结果进行比较。制备了两种不同的离子交换柱来分离和纯化每个白蛋白样品，并对通过这两种柱洗脱的物质进行了鉴定。肾病洗脱物质，特别是在复发状态下，与正常受试者的洗脱物质存在一些差异。通过圆二色光谱估计的α-螺旋含量发现，缓解期比复发期更接近正常白蛋白。通过计算结合参数、解离常数和结合位点数量，研究了荧光探针1-苯胺基-8-萘磺酸盐与白蛋白的结合。缓解期的结合参数比复发期更接近正常白蛋白的结合参数。
