Takamatsu S, Horie T, Mishima T, Minamide Y, Hayashi M, Awazu S
Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
Biochem Pharmacol. 1990 Apr 1;39(7):1205-12. doi: 10.1016/0006-2952(90)90264-l.
Rat plasma albumin fractionated by Sephadex G-75 (superfine) gel filtration from freshly prepared rat plasma was further separated into three major fractions by chromatofocusing column chromatography. All the fractionated albumins had high binding affinity for the fluorescent probe 8-anilino-1-naphthalenesulfonic acid, as the model compound for acidic drugs, and were found to be immunologically identical.
通过葡聚糖凝胶G-75（超细）凝胶过滤法从新鲜制备的大鼠血浆中分离得到的大鼠血浆白蛋白，再经色谱聚焦柱色谱法进一步分离为三个主要组分。所有分离得到的白蛋白对作为酸性药物模型化合物的荧光探针8-苯胺基-1-萘磺酸具有高结合亲和力，并且在免疫学上是相同的。
