Brown P J, Johnson P M
J Reprod Immunol. 1981 Dec;3(6):351-8. doi: 10.1016/0165-0378(81)90051-6.
Intact syncytiotrophoblast microvillous plasma membrane (StMPM) vesicle preparations isolated from human term placentae express surface Fc gamma receptor activity and will bind to Sepharose-immobilised human IgG. This IgG-StMPM Fc gamma receptor interaction has been shown to be stable during de-membranation using the anionic detergent 1% sodium deoxycholate (NaDOC) and to be preferentially contained in an NaDOC-resistant fraction of StMPM vesicles. Following NaDOC treatment of StMPM adsorbed on to Sepharose-IgG, subsequent desorption with the chaotrope 0.5 M NH4SCN disrupted the IgG-Fc gamma receptor interaction. The NH4SCN desorption product, however, contained several molecular components including IgG, transferrin and actin. These results indicate that StMPM surface Fc gamma receptors may interact with proteins of the microfilamentous core of StMPM vesicles and also possibly be topographically associated with some other cell surface components.
从足月人胎盘分离出的完整合体滋养层微绒毛质膜（StMPM）囊泡制剂表现出表面Fcγ受体活性，并能与固定在琼脂糖上的人IgG结合。已证明这种IgG - StMPM Fcγ受体相互作用在使用阴离子去污剂1%脱氧胆酸钠（NaDOC）进行去膜处理时是稳定的，并且优先包含在StMPM囊泡的耐NaDOC部分中。在用NaDOC处理吸附在琼脂糖 - IgG上的StMPM后，随后用离液剂0.5 M NH4SCN进行解吸破坏了IgG - Fcγ受体相互作用。然而，NH4SCN解吸产物包含几种分子成分，包括IgG、转铁蛋白和肌动蛋白。这些结果表明，StMPM表面Fcγ受体可能与StMPM囊泡微丝核心的蛋白质相互作用，并且也可能在拓扑结构上与一些其他细胞表面成分相关。
