人合体滋养层微绒毛质膜的免疫球蛋白G和转铁蛋白受体

The IgG and transferrin receptors of the human syncytiotrophoblast microvillus plasma membrane.

作者信息

Johnson P M, Brown P J

出版信息

Am J Reprod Immunol (1980). 1980;1(1):4-9. doi: 10.1111/j.1600-0897.1980.tb00003.x.

DOI:10.1111/j.1600-0897.1980.tb00003.x

Abstract

Fc gamma receptor activity has been demonstrated using a radioisotopic assay on human syncytiotrophoblast microvillus plasma membrane (StMPM) vesicle preparations isolated from normal full-term placentas. Prior chaotropic extraction to remove extrinsic membrane protein from StMPM preparations was shown to increase radiolabeled IgG uptake by up to 30%. Polyacrylamide disc gel electrophoresis (PAGE) has been used to indicate that the StMPM receptor for transferrin solubilizes in 1% sodium deoxycholate and retains its binding activity for transferrin. Quantitative data for both Fc gamma and transferrin receptors obtained following study of isolated StMPM preparations are summarized. Current concepts for the potential role of trophoblast Fc gamma and transferrin receptors in the mechanisms of transplacental passage of IgG and iron, respectively, from mother to fetus are illustrated and discussed.

摘要

已通过放射性同位素分析法，对从正常足月胎盘分离出的人合体滋养层微绒毛质膜（StMPM）囊泡制剂进行检测，证实了Fcγ受体活性。结果显示，事先用离液剂提取以去除StMPM制剂中的外在膜蛋白，可使放射性标记的IgG摄取量增加多达30%。聚丙烯酰胺圆盘凝胶电泳（PAGE）已用于表明，转铁蛋白的StMPM受体可在1%脱氧胆酸钠中溶解，并保留其对转铁蛋白的结合活性。总结了对分离出的StMPM制剂进行研究后获得的Fcγ和转铁蛋白受体的定量数据。阐述并讨论了目前关于滋养层Fcγ和转铁蛋白受体分别在IgG和铁从母体向胎儿经胎盘转运机制中潜在作用的概念。